primary antibodies against hdac2  (Cell Signaling Technology Inc)

Journal: Virology Journal

Article Title: Histone deacetylase III interactions with BK polyomavirus large tumor antigen may affect protein stability

doi: 10.1186/s12985-023-02128-6

Figure Lengend Snippet: HDAC3 and HDAC8 are required for BKPyV LT protein expression. A The expression of class I HDACs was knocked down by siRNA, then cells were infected with BKPyV and LT protein expression was determined by immunofluorescence and flow cytometric analysis 48 h post-infection. Middle panel: Quantification of the LT expression levels in the immunofluorescence assay. Right panel: LT protein expression was determined by intracellular staining and quantified by flow cytometry. B Class I HDACs were inhibited by HDAC inhibitors, cells were infected with BKPyV, and LT protein expression was determined by immunofluorescence and flow cytometric analysis 48 h postinfection. Middle panel: Quantification of the LT expression levels in the immunofluorescence assay. Right panel: LT protein expression was determined by intracellular staining and quantified by flow cytometry. BKPyV infection without siRNA or HDAC inhibitor treatment was considered 100%. (Green, LT protein; Red, Evan’s Blue stain). Data are representative of at least three independent experiments and are shown as the mean ± SD (* P < 0.05; ** P < 0.01; *** P < 0.001). (MFI: Mean fluorescence intensity)

Article Snippet: Primary antibodies against HDAC1, HDAC2, HDAC3, and HDAC8 were purchased from Cell Signaling Technology Inc. (Danvers, Massachusetts, USA).

Techniques: Expressing, Infection, Immunofluorescence, Staining, Flow Cytometry, Fluorescence

Journal: Aging (Albany NY)

Article Title: Profiling and integrated analysis of transcriptional addiction gene expression and prognostic value in hepatocellular carcinoma

doi: 10.18632/aging.204676

Figure Lengend Snippet: Literature review identified the transcriptional addiction genes.

Article Snippet: We incubated the tissue microarrays with the primary antibody against HDAC2 (Servicebio, GB11371, Wuhan), followed by the secondary antibody (Servicebio, GB23303, Wuhan) and 3,3’-diaminobenzidine (DAB) IHC kit (DAKO, K5007, Denmark).

Techniques:

Journal: Aging (Albany NY)

Article Title: Profiling and integrated analysis of transcriptional addiction gene expression and prognostic value in hepatocellular carcinoma

doi: 10.18632/aging.204676

Figure Lengend Snippet: Each genetic parameter of the risk signature.

Article Snippet: We incubated the tissue microarrays with the primary antibody against HDAC2 (Servicebio, GB11371, Wuhan), followed by the secondary antibody (Servicebio, GB23303, Wuhan) and 3,3’-diaminobenzidine (DAB) IHC kit (DAKO, K5007, Denmark).

Techniques:

Journal: Aging (Albany NY)

Article Title: Profiling and integrated analysis of transcriptional addiction gene expression and prognostic value in hepatocellular carcinoma

doi: 10.18632/aging.204676

Figure Lengend Snippet: Expression levels of HDAC2 in different tissues. ( A ) HDAC2 expression in normal and tumor tissues in the TCGA-LIHC cohort. ( B ) HDAC2 expression in normal and tumor tissues in the ICGC-JP cohort. ( C ) The protein level of HDAC2 in normal and tumor tissues in HPA database. ( D ) The protein levels of HDAC2 in tumor tissue, paracancerous tissue, and distal tissue in the tissue microarray.

Article Snippet: We incubated the tissue microarrays with the primary antibody against HDAC2 (Servicebio, GB11371, Wuhan), followed by the secondary antibody (Servicebio, GB23303, Wuhan) and 3,3’-diaminobenzidine (DAB) IHC kit (DAKO, K5007, Denmark).

Techniques: Expressing, Microarray

Journal: Aging (Albany NY)

Article Title: Profiling and integrated analysis of transcriptional addiction gene expression and prognostic value in hepatocellular carcinoma

doi: 10.18632/aging.204676

Figure Lengend Snippet: Relationship between HDAC2 expression and patient survival. ( A ) Kaplan-Meier survival analysis of patients with different HDAC2 expressions in TCGA-LIHC cohort. ( B ) Kaplan-Meier survival analysis of patients with different HDAC2 expressions in ICGC-JP cohort. ( C ) Scored for each sample of the tissue microarray. ( D , E ) Kaplan-Meier survival analysis of patients with different tissue scores in the tissue microarrays.

Article Snippet: We incubated the tissue microarrays with the primary antibody against HDAC2 (Servicebio, GB11371, Wuhan), followed by the secondary antibody (Servicebio, GB23303, Wuhan) and 3,3’-diaminobenzidine (DAB) IHC kit (DAKO, K5007, Denmark).

Techniques: Expressing, Microarray

Journal: Aging (Albany NY)

Article Title: Profiling and integrated analysis of transcriptional addiction gene expression and prognostic value in hepatocellular carcinoma

doi: 10.18632/aging.204676

Figure Lengend Snippet: Correlation of HDAC2 score with HCC pathological characteristics in the tissue microarray set. ( A ) HDAC2 score in tumor recurrence and non-recurrence groups. ( B ) HDAC2 score in tumor metastatic and non-metastatic groups. ( C ) HDAC2 score in different tumor size groups. ( D ) HDAC2 score in different tumor stage groups. ( E , F ) Univariate and multivariate regression analyses for HDAC2 score as an independent prognostic factor.

Article Snippet: We incubated the tissue microarrays with the primary antibody against HDAC2 (Servicebio, GB11371, Wuhan), followed by the secondary antibody (Servicebio, GB23303, Wuhan) and 3,3’-diaminobenzidine (DAB) IHC kit (DAKO, K5007, Denmark).

Techniques: Microarray

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Histone Deacetylase 2 Suppresses Skeletal Muscle Atrophy and Senescence via NF-κB Signaling Pathway in Cigarette Smoke-Induced Mice with Emphysema

doi: 10.2147/COPD.S314640

Figure Lengend Snippet: Overexpression of HDAC2 Inhibits Senescence in C2C12 Cells. ( A ) β-Galactosidase staining measurement of cellular senescence after 24 hours of 0.3% CSE between the different groups, 100×. ( B ) The protein expression level of P53, P21, SMP30 in cells with or without 0.3% CSE. ( C ) The mRNA level of P53, P21 in cells with or without 0.3% CSE. ( D ) The protein expression level of NF-κBp65 and IKK in cells with or without 0.3% CSE. Values are expressed as means±SD. Experiments were repeated 3 times with similar results. * p <0.05 vs control NC group (LV-NC), # p <0.05 vs CSE+LV-NC group.

Article Snippet: The membranes were then incubated with primary antibodies against HDAC2, P53, P21, IKK, and NF-κBp65 (1:1000, Cell Signaling Technology, USA) and MURF1, MAFbx, and SMP30 (1:1000, Abcam, UK) overnight at 4°C, followed by incubation with fluorescent secondary antibodies (1:1000, Cell Signaling Technology, USA).

Techniques: Over Expression, Staining, Expressing, Control

Journal: International Journal of Chronic Obstructive Pulmonary Disease

Article Title: Histone Deacetylase 2 Suppresses Skeletal Muscle Atrophy and Senescence via NF-κB Signaling Pathway in Cigarette Smoke-Induced Mice with Emphysema

doi: 10.2147/COPD.S314640

Figure Lengend Snippet: Effects of NF-κB pathway on HDAC2-regulated atrophy and senescence in C2C2 cells. ( A ) Western blot assays for NF-κBp65 expression after overexpression of HDAC2 and PDTC in CSE. ( B ) Immunofluorescence detection of myotube diameter, 100×. ( C ) Western blot assays for MURF1, MAFbx, P53, and P21. Values are expressed as means±SD. Experiments were repeated 3 times with similar results. * p <0.05 vs CSE group, # p <0.05 vs CSE+LV-HDAC2 group, $ p <0.05 vs CSE+PDTC group.

Article Snippet: The membranes were then incubated with primary antibodies against HDAC2, P53, P21, IKK, and NF-κBp65 (1:1000, Cell Signaling Technology, USA) and MURF1, MAFbx, and SMP30 (1:1000, Abcam, UK) overnight at 4°C, followed by incubation with fluorescent secondary antibodies (1:1000, Cell Signaling Technology, USA).

Techniques: Western Blot, Expressing, Over Expression, Immunofluorescence